UCR

Center for Plant Cell Biology



Members


Venugopala Reddy GonehalVenugopala Reddy Gonehal

Associate Professor

Mailing Address:

Botany and Plant Sciences
Genomics Building /4234C
University of California
Riverside, CA 92521

Phone: (951) 827-3482
Fax: (951) 827-4437
Email: venu.gonehal@ucr.edu

Degree(s):

PhD 1999 Tata Institute of Fundamental Research, Mumbai, India
MS 1994 Univ. of Agricultural Sciences, Bangalore, India
BSc 1991 Univ. of Agricultural Sciences, Bangalore, India

College/Division Affiliation:

College of Natural and Agricultural Sciences

Center/Inst Affiliation(s):

Center for Plant Cell Biology

Areas Of Expertise:

Gene Networks; Live Imaging; Stem Cells; Development

Awards / Honors:

1999-02 Fellow of the Jane Coffin Childs Memorial Fund for Medical Research

Research Summary:

We are interested in exploring the molecular and cellular basis of cell identity transition. The process of cell identity transition involves conversion of transient temporal signals into stable genetic circuits that involve feed-forward and feedback control of gene activation. Therefore, we employ dynamic methods of transient perturbations, in cell-cell communication, followed by cell-type specific genomics and live-imaging to obtain a dynamic view of molecular and cellular architecture that dictate cell identity transitions.



Structure of stem-cell niche highlighted by fluorescent reporter constructs.
(A), (B) and (D) are the 3-D re-constructed views of shoot apical meristems (SAMs), highlighting the stem-cell domain (A), the stem-cell daughters begin to differentiate in the peripheral zone (PZ) (B and D) and also within the Rib-meristem (RM) (C). (E-G) are the re-constructed side views of SAMs represented in (A-C) respectively, revealing 3 clonal layers of cells (E,F) and RM (G).  Cell outlines are highlighted by FM4-64 dye (red).

The shoot apical meristem stem-cell niche provides an ideal developmental gradient to explore the meaning of transient cell identity at molecular, cellular and tissue level.  The SAM stem-cell niche of Arabidopsis thaliana is a dynamic structure consisting of distinct cell types which exhibit specific gene activities and cellular behaviors. The central zone (CZ) hosts a set of stem-cells. Progeny of stem-cells differentiate within the surrounding peripheral zone (PZ). The CZ also supplies cells to the rib-meristem (RM) region located beneath the CZ where cells differentiate and become part of the stem. The dynamic analysis of SAM stem-cell niche might provide clues to the regulation of molecular code that defines stem-cell identity and also the regulation of cell identity transition in general.

Our work focuses on two main areas:

  1. Defining the molecular code and its regulation, at genome scale, for distinct cell types of the SAM stem-cell niche.  We are employing various methods of enrichment of specific cell types and genomic technologies to achieve this goal.
  2. Transforming the molecular code into spatial maps representing functional interactions within living SAMs by utilizing dynamic live-imaging methods.

Related Press Releases:

Selected Publications:

List of publications from HubMed

Lab Personnel:

Yadav, Ram-Kishore
Postdoctoral Researcher—  Genomics, Live-imaging
Perales, Mariano
Postdoctoral Researcher —
Xie, Mingtang
Postdoctoral Researcher —
Snipes, Andy
Graduate Student Researcher —
Tataw, Moses
Graduate Student Researcher —

 

More Information

General Campus Information

University of California, Riverside
900 University Ave.
Riverside, CA 92521
Tel: (951) 827-1012

Career OpportunitiesUCR Libraries
Campus StatusDirections to UCR

Center Information

Center for Plant Cell Biology
Botany & Plant Sciences Department
2150 Batchelor Hall

Tel: (951) 827-7177
Fax: (951) 827-5155
E-mail: genomics@ucr.edu

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