Xuemei Chen, Robert Jinkerson, and Meng Chen received an NSF EAGER grant

By AImee Gonzales |

Xuemei Chen, Robert Jinkerson, and Meng Chen received an NSF grant to establish a transformative RNA sequencing technology for studying plastids.


The plant cell stores its DNA in not only the nucleus but also the plant-specific organelles, the plastids. Plastid DNA can be transcriptionally programmed to instruct the differentiation of plastids into diverse types, such as the well-known photosynthetically active chloroplasts. In fact, plastids function far beyond photosynthesis in a variety of essential roles in development, metabolism, signaling, and immunity in plants. However, because each plant cell harbors tens to hundreds of plastids, current RNA sequencing approaches – even those using single cells – average plastid transcriptomic profiles, and thereby, potentially obscure biologically relevant transcriptomic variations that distinguish unique plastid types. As a result, our understanding of plastid types and their functions remains rudimentary.

A UCR team consisting of IIGB faculty Xuemei Chen, Robert Jinkerson, and Meng Chen have been awarded an NSF EAGER grant to tackle the long-standing problem in plant biology. The NSF EAGER (Early-concept Grants for Exploratory Research) mechanism supports radically different and potentially transformative research ideas or approaches. The two-year grant of $300,000, entitled “spRNA-seq: high-throughput transcriptome analysis of single plastids”, is aimed at establishing a cutting-edge technology to sequence RNA molecules from single plastids, namely single-plastid RNA sequencing (spRNA-seq). spRNA-seq allows the determination of molecular signatures of thousands of individual plastids in one experiment, making it possible to create a complete plastid-type atlas and elucidate their functions. This enabling technology is expected to revolutionize research on plastids and will generate novel insights into how plastids affect plant biology, ecology, and evolution.

Let us help you with your search